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为探讨人疱疹病毒６型（ＨＨＶ－６）感染对单核／巨噬细胞功能的影响，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）和双抗体夹心ＥＬＩＳＡ法检测ＩＬ－１０及ＩＬ－１２的产生。ＨＨＶ－６诱导单核细胞、ＴＨＰ－１细胞表达及产生ＩＬ－１０和ＩＬ－１２。细胞因子ｍＲＮＡ动力学观察显示ＩＬ－１２随ＩＬ－１０ｍＲＮＡ积累而减少，用抗人ＩＬ－１０的单克隆抗体阻断ＨＨＶ－６诱导的内源性ＩＬ－１０，ＩＬ－１２ｍＲＮＡ表达和因子产生明显增加，提示内源性ＩＬ－１０抑制ＩＬ－１２产生。在ＨＨＶ－６感染培养中加入ＩＦＮ－γ，ＩＬ－１２基因表达随ＩＦＮ－γ浓度而增加，ＩＬ－１０ｍＲＮＡ测减少，ＩＬ－１２和ＩＬ－１０因子测定结果亦明显增加或降低，表明ＩＦＮ－γ在转录水平调节ＨＨＶ－６诱导的ＩＬ－１２和ＩＬ－１０产生，其机理可能主要与ＩＦＮ－γ抑制内源性ＩＬ－１０有关。
To investigate the effect of HHV-6 infection on the function of monocytes / macrophages, the expressions of IL-10 and IL-10 were detected by reverse transcription polymerase chain reaction (RT-PCR) and double antibody sandwich ELISA 12 generation. HHV-6 induces monocyte, THP-1 cell expression and production of IL-10 and IL-12. Cytokine mRNA kinetics showed that IL-12 decreased with the accumulation of IL-10 mRNA. Anti-IL-10 monoclonal antibody blocked HHV-6-induced endogenous IL-10 and IL-12 mRNA expression and factor production significantly Increasing, suggesting that endogenous IL-10 inhibits IL-12 production. The expression of IL-12 increased with the concentration of IFN-γin HHV-6 infected culture, the decrease of IL-10mRNA and the increase or decrease of IL-12 and IL-10 also showed that IFN- γ regulates the production of IL-12 and IL-10 induced by HHV-6 at the transcriptional level, and its mechanism may be mainly related to the inhibition of endogenous IL-10 by IFN-γ.