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【目的】研究耐旱性高的红麻品种在干旱胁迫条件下的蛋白质表达,揭示红麻耐旱性的生理机制。【方法】以鉴定出的耐旱性红麻品种GA42为材料,在苗期(五叶期)设置正常供水与控水比较试验,运用双向电泳分析红麻在干旱胁迫和正常供水条件下叶片蛋白质组的动态变化。【结果】对2-DE图谱分析后发现,在干旱胁迫下出现65个差异表达蛋白质点,选用表达量明显上调的9个蛋白质点,通过MALDI-TOF-TOF MS分析和数据库检索,鉴定出6个差异表达蛋白的功能,分别是2个核酮糖-1,5-二磷酸羧化酶(Rubisco)或其大亚基(所有植物进行光合碳同化的关键酶)、1个Rubisco活化酶(广泛存在于植物中调节Rubisco活性的酶)、1个二甲基萘醌甲基转移酶(一种参与甲基转移反应的辅酶)、1个推定的胞质型谷氨酰胺合成酶(参与高等植物氨同化过程的关键酶)、1个ATP合酶β亚基(在活性细胞中起着将其它能量合成为生物能量通货-ATP的能量转换作用)。【结论】揭示了红麻GA42表现出较强的耐旱性与上述6个差异表达蛋白质点明显上调有关。
【Objective】 The objective of this study was to investigate the protein expression of drought-tolerant kenaf under drought stress and reveal the physiological mechanism of drought tolerance in kenaf. 【Method】 Based on the identification of drought tolerance kenaf GA42, the normal water supply and water control were set up at the seedling stage (five leaf stage). Two-dimensional electrophoresis was used to analyze the leaf protein content of kenaf under drought stress and normal water supply Group dynamics. 【Result】 The result of 2-DE analysis showed that there were 65 differentially expressed protein spots under drought stress and 9 protein spots with significantly up-regulated expression level were selected. MALDI-TOF-TOF MS analysis and database search identified 6 The function of differentially expressed proteins were 2 Rubisco or its large subunit (the key enzyme for photosynthetic carbon assimilation in all plants) and 1 Rubisco activase An enzyme widely present in plants that regulates Rubisco activity), 1 dimethylnaphthaquinone methyltransferase (a coenzyme involved in the methyl transfer reaction), 1 putative cytoplasmic glutamine synthetase (involved in higher A key enzyme in the plant ammonia assimilation process) and one ATP synthase beta subunit, which plays a role in the energy conversion of other energy into bioenergetic currency-ATP. 【Conclusion】 The results showed that kenaf GA42 showed strong drought tolerance and the above six differentially expressed proteins were significantly up-regulated.