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通过对粳籼89的穗颈瘟分离菌株的致病力及遗传宗谱研究,结果表明:(1)粳籼89分离菌株的小种类型复杂,既有籼型小种如ZA1、ZB1、ZB5、ZB13、ZC13等,又有粳型小种如ZD5、ZG1等;(2)该类菌株的致病力存在不稳定性,同一菌株不同时间接种,鉴别寄主上表现的小种类型不同,对其他主栽水稻品种或抗源的致病力也表现不同,但这些菌株无论何时接种到粳籼89上均能使其表现感病,病级在4级以上;(3)该类菌株接种到粳籼89衍生品种或其他粳籼杂交后代上,一般能侵染这类品种;(4)利用RFLP技术,采用探针MGR586与限制性内切酶EcoRI组合对病菌进行DAN指纹分析,结果16个粳籼89分离菌株被分在2个相邻的遗传宗谱里,即宗谱1(9个菌株)和宗谱2(7个菌株),这两个宗谱恰好是广东的优势宗谱。
The results showed that: (1) The small-breed type of japonica-japonica 89 isolates was complicated, including indica-type races such as ZA1, ZB1 and ZB5 , ZB13, ZC13 and so on, as well as japonica races such as ZD5 and ZG1. (2) The pathogenicity of these strains is not stable. The same strains were inoculated at different times to identify the different types of races on which the host presented. The virulence of other main rice cultivars or anti-sources also showed different performance, but these strains could make them susceptible to inoculation whenever they were inoculated into japonica rice 89, and the disease grade was above grade 4. (3) (4) RF fingerprinting (MGR586) and restriction endonuclease (EcoRI) were used to analyze the fingerprints of the bacteria by RFLP technique. The results showed that 16 The isolates of japonica-japonica 89 were divided into two adjacent genealogies, namely, genealogy 1 (9 strains) and genealogy 2 (7 strains), which were exactly the dominant genealogies in Guangdong.