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本文以配体N,N′-二(3-氨丙基)-4-甲氧基苄胺(amba)与4,4′-联吡啶,铜盐反应合成了两种多胺Cu(Ⅱ)配合物[Cu(amba)Cl2](1)和[Cu(4,4′-bipy)(amba)(ClO4)]ClO4(2),通过红外光谱、质谱和X-射线单晶衍射对其结构进行了表征。利用紫外吸收光谱、分子荧光,电化学以及粘度试验研究了配合物与小牛胸腺DNA(CT-DNA)的相互作用方式。通过紫外吸收光谱得到配合物1和2与DNA的结合常数Kb分别为1.3×104 mol-1.L和1.7×104 mol-1.L,荧光光谱得到配合物1和2的荧光淬灭常数分别为1.04×103mol-1.L和1.81×103 mol-1.L,表明了配合物1和2与CT-DNA结合方式均为静电模式。凝胶电泳实验的结果表明配合物对pBR322 DNA的切割均为水解切割。
In this paper, two polyamines Cu (Ⅱ) were synthesized by the reaction of the ligand N, N’-bis (3-aminopropyl) -4-methoxybenzylamine (amba) The structures of the complexes [Cu (amba) Cl2] (1) and [Cu (4,4’-bipy) amba (ClO4)] ClO4 (2) were characterized by IR, MS and X- Characterized. The interaction between the complex and calf thymus DNA (CT-DNA) was studied by ultraviolet absorption spectroscopy, molecular fluorescence, electrochemical and viscosity tests. The binding constants Kb of complexes 1 and 2 with DNA were 1.3 × 104 mol-1L and 1.7 × 104 mol-1L, respectively. The fluorescence quenching constants of complex 1 and 2 were obtained by UV spectroscopy 1.04 × 103mol-1.L and 1.81 × 103mol-1.L, indicating that complexes 1 and 2 and CT-DNA binding mode are electrostatic mode. The results of gel electrophoresis showed that the cleavage of the pBR322 DNA by the complex was hydrolytic cleavage.