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目的和方法 :以高脂血清作用于培养的血管内皮细胞 ,通过检测内皮细胞表面粘附分子ICAM- 1的表达及培养液中NO-2 的含量 ,研究复合酶Ⅱ抗高脂血清所致细胞损伤的保护效应。结果 :高脂血清可使培养的血管内皮细胞表面粘附分子ICAM - 1的表达显著增加 (由 0 2 2 8± 0 0 2 5增加到 0 32 8± 0 0 30 ,P <0 0 1) ,细胞合成和释放NO-2 的含量明显减少 (由 0 433± 0 144减少到 0 149± 0 0 34 ,P <0 0 1) ;而复合酶Ⅱ则能显著降低ICAM - 1的表达 (0 2 2 9± 0 0 6 5 ) ,增加内皮细胞NO-2 的合成与释放 (0 386± 0 0 36 ) ,与高脂血清损伤组相比 ,差异有高度显著性 (P <0 0 1)。结论 :复合酶Ⅱ同时具有SOD和CAT活性结构和作用特性 ,能更有效地清除氧自由基 ,对高脂血清造成的血管内皮细胞损伤具有明显的抗损伤效应
Aims and Methods: The effect of compound enzyme Ⅱ on hyperlipidemic serum-induced vascular endothelial cells was studied by detecting the expression of ICAM-1 on endothelial cell surface and the content of NO-2 in culture medium. The protective effect of injury. Results: The expression of ICAM - 1 on the surface of cultured vascular endothelial cells was significantly increased by high - fat serum (from 0228 ± 0 025 to 0328 ± 0 030, P <0 01) , The content of NO-2 in cell synthesis and release decreased significantly (from 0 433 ± 0 144 to 0 149 ± 0 0 34, P <0.01), whereas compound enzyme Ⅱ significantly reduced the expression of ICAM-1 (P <0.01). Compared with the hyperlipidemia group, the difference was significant (P <0.01). The level of NO-2 production in endothelial cells was significantly increased . CONCLUSION: The complex enzyme Ⅱ possesses both the structure and the action characteristics of SOD and CAT activity, which can scavenge oxygen free radicals more effectively and has obvious anti-injury effect on vascular endothelial cell injury caused by high-fat serum