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? Objective:To explore the molecular mechanism of G1arrest of thymocytes and splenocytes in mice induced by whole body irradiation(WBI) .Methods:Flow cytometry(FCM) and dotblotwere used to analyze MDM2 m RNA and protein expressions of thymocytes and splenocytes in Kunming mice after whole body irradiation (WBI) with x- rays.Results:The expressions of MDM2 protein of thymocytes in mice increased from 4h to 8h after 2 Gy WBI.There were no significant changes of MDM2 m RNA level of thymocytes and splenocytes from0 to48h after75m Gy or2 Gy WBI.No changes of MDM2 m RNA level were also found in thymocytes or splenocytes of mice4h after0～ 6Gy WBI.Conclusion:These results suggest that ionizing radiation could induce MDM2 protein expression and make G1arrest cells reentering cell cycle. 〔
Objective: To explore the molecular mechanism of G1 arrest of thymocytes and splenocytes in mice induced by whole body irradiation (WBI). Methods: Flow cytometry (FCM) and dot blotting used to analyze MDM2 m RNA and protein expressions of thymocytes and splenocytes in Kunming mice after whole body irradiation (WBI) with x-rays. Results: The expressions of MDM2 protein of thymocytes in mice increased from 4h to 8h after 2 Gy WBI. There were no significant changes of MDM2 m RNA level of thymocytes and splenocytes from 0 to 48h after 75m Gy or2 Gy WBI. Novel changes of MDM2 m RNA level were also found in thymocytes or splenocytes of mice 4h after 0 ~ 6Gy WBI.Conclusion: These results suggest that ionizing radiation could induce MDM2 protein expression and make G1arrest cells reentering cell cycle. [