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背景:神经营养因子在神经系统发育和正常生理功能维持及神经损伤中起着重要的作用。对神经营养因子的研究是目前神经科学领域的热点和前沿课题之一。目的:研究脑室内注射脑源性神经营养因子(brain-derivedneurotrophicfactor,BDNF)抗体阻断内源性BDNF对大鼠海马一氧化氮合酶(nitricoxidesynthase,NOS)阳性神经元数目的影响。设计:随机对照的实验研究。地点和对象:实验地点在中山大学基础医学院脑研究室。实验对象为健康雄性SD大鼠13只。干预:大鼠脑室内注射BDNF抗体一周后,采用Morris水迷宫进行行为检测。主要观察指标:观察大鼠定位航行试验和空间探索试验状况,并用NADPH-黄递酶组化染色方法观察海马NOS阳性神经元数目的变化。结果:定位航行试验:实验组大鼠平均逃避潜伏期为(33.46±2.64)s,对照组为(17.71±1.86)s,两者差异具有非常显著性意义(t=4.8733,P<0.01);空间探索试验:实验组大鼠在平台象限游泳距离百分比为(28.89±6.31)%,对照组为(41.99±7.46)%,实验组明显低于对照组(t=3.3907,P<0.01);与对照组相比,实验组大鼠空间学习和记忆能力明显下降。实验组大鼠海马CA1区NOS阳性神经元数目(38.37±5.23)明显少于对照组(49.53±5.74)(t=8.200,P<0.01);实验组DG区NOS阳性神经元数目(48.77±5.51)明显少于
Background: Neurotrophic factors play an important role in the maintenance of the nervous system and in the maintenance of normal physiological functions and nerve damage. Neurotrophic factor research is currently one of the hot topics in the field of neuroscience. Objective: To investigate the effects of intraventricular injection of brain-derived neurotrophic factor (BDNF) antibody on the number of nitric oxide synthase (NOS) positive neurons in hippocampus of rats after blocking endogenous BDNF. Design: Randomized controlled experimental study. Venue and Subject: The experimental site was in Brain Research Laboratory, School of Basic Medicine, Sun Yat-sen University. Thirteen healthy male SD rats were selected as experimental subjects. Intervention: After one week of intracerebroventricular injection of BDNF antibody in rats, the Morris water maze was used for behavioral testing. MAIN OUTCOME MEASURES: The positioning navigation test and space exploration test were performed in rats. The changes of NOS positive neurons in hippocampus were observed by NADPH-diaphorase histochemical staining. Results: The positioning navigation test showed that the mean escape latency of experimental group was (33.46 ± 2.64) s and that of control group was (17.71 ± 1.86) s, the difference between the two groups was significant (t = 4.8733, P <0.01) Exploration test: The swimming distance of the experimental group in the platform quadrant was (28.89 ± 6.31)%, the control group was (41.99 ± 7.46)%, the experimental group was significantly lower than the control group (t = 3.3907, P <0.01) Compared with the control group, the spatial learning and memory abilities of the experimental group decreased significantly. The number of NOS positive neurons in hippocampal CA1 region in experimental group was significantly lower than that in control group (38.37 ± 5.23) (49.53 ± 5.74) (t = 8.200, P <0.01). The number of NOS positive neurons in experimental group was 48.77 ± 5.51 ) Is significantly less than