论文部分内容阅读
生长素应答因子(Auxin responsive factors)是植物内传导生长素信号、调控生长素响应的功能基因的表达过程中,起中心作用的重要转录因子,参与植物生长发育的各个方面的调控,包括组织分化、器官发生、顶端优势和根生成、趋向性等。我们利用豆科植物的模式作物,蒺藜苜蓿已有的全基因组序列数据,应用生物信息学的手段,对蒺藜苜蓿全基因组和天蓝苜蓿叶片转录组ARFs基因家族进行系统的分析和基因功能预测。共鉴定出34个完整的蒺藜苜蓿ARFs基因,其中27个基因有基因芯片的表达证据。天蓝苜蓿鉴定出11个完整的ARFs基因。染色体定位分析发现在蒺藜苜蓿第5染色体上聚集了6个基因结构和氨基酸序列均非常相似的ARFs。系统进化分析表明这6个基因在拟南芥中没有同源基因。拟南芥的部分ARFs在苜蓿中也没有同源基因。应用基因芯片数据进行基因表达分析表明蒺藜苜蓿的ARFs在不同器官特异性的表达。部分蒺藜苜蓿ARFs成员响应盐胁迫和菌根真菌侵染。Medtr8g100050不仅响应盐胁迫上调表达,菌根真菌侵染后也上调表达。
Auxin responsive factors are important transcription factors that play a central role in the process of the expression of functional genes that regulate the auxin response in plants. They are involved in the regulation of various aspects of plant growth and development, including tissue differentiation , Organogenesis, top superiority and root formation, tendencies and so on. Based on the genome-wide sequence data of Medicago truncatula, a model crop of legumes, bioinformatics methods were used to systematically analyze and predict the gene function of the ARFs gene family of Medicago truncatula genome and leaves of Azure blue clover. A total of 34 intact Medicago truncatula ARFs were identified, of which 27 were gene chip evidence of expression. Sky blue alfalfa identified 11 complete ARFs genes. Chromosome localization analysis revealed that there were 6 ARFs with similar gene structure and amino acid sequence on Medicago truncatula chromosome 5. Phylogenetic analysis showed that these six genes did not have homologous genes in Arabidopsis. Some Arabidopsis ARFs also have no orthologs in alfalfa. Gene expression analysis using gene chip data showed that ARFs from Medicago truncatula expressed differently in different organs. Some members of Medicago truncatula ARFs responded to salt stress and mycorrhizal fungal infection. Medtr8g100050 not only up-regulated the expression of salt stress, but also up-regulated the expression of mycorrhizal fungi after infection.