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背景:以往关于口腔综合治疗台水路细菌污染状况的研究较多,但口腔专科医院不同科室间因业务内容不同,其对口腔综合治疗台高速涡轮机水路的影响如何尚未见报道。目的:评估口腔专科医院不同专业各科室口腔综合治疗台高速涡轮机水路的细菌污染状况,以期为各科室制定个性化的感染控制措施提供依据。设计、时间及地点:横断面调查,于2007-05/2008-03完成实验及数据整理,取样于广东省口腔医院,细菌学检测于南方医科大学公共卫生与热带医学学院微生物实验室完成。材料:普通营养琼脂、血平板、微生物生化反应鉴定系统、链球菌凝集试验试剂盒、革兰染色液等购自广东环凯微生物试剂公司。方法:广东省口腔医院80张口腔综合治疗台接无菌高速涡轮机后取水样,取样时间为2007-05/10隔周星期五下班后,每机每次取6mL,共取10次。采用常规微生物分离鉴定技术进行细菌学检测。主要观测指标:水样中细菌浓度和葡萄球菌、链球菌、放线菌3种细菌的检出率。结果:口腔医院各临床科室口腔综合治疗台高速涡轮机水路水样中细菌浓度5.15×10～1.59×103cfu/mL,平均5.67×102cfu/mL,牙周病科水样细菌浓度最高,口腔颌面外科水样细菌浓度最低,各科室间差异有显著性意义(P=0.000);各科室间水样3种细菌的检出率差异亦有显著性意义(P=0.000)。结论:口腔专科医院不同科室的口腔综合治疗台高速涡轮机水路细菌污染状况不同,对于高速涡轮机使用频率较低的科室建议将空踩口腔综合治疗台高速涡轮机水路放水列入每天工作常规,而对于临床业务污染较严重的科室还应考虑采用额外的感染控制措施。
BACKGROUND: In the past, there were many researches on the bacterial contamination of the waterways of oral integrated treatment stations. However, how the influence of different departments in the oral special hospitals on the high speed turbine waterway of the oral comprehensive treatment units has not been reported yet. OBJECTIVE: To evaluate the bacterial contamination of the high-speed turbine waterways in the oral comprehensive treatment units in various departments of the Oral Specialized Hospital in order to provide the basis for the development of personalized infection control measures in various departments. DESIGN, TIME AND SETTING: Cross-sectional survey was conducted to complete the experiment and data collection from 2007-05 / 2008-03. Samples were collected from Guangdong Stomatological Hospital and bacteriological tests were performed at the Microbiology Laboratory, School of Public Health and Tropical Medicine, Southern Medical University. Materials: General nutrition agar, blood plate, biochemical reaction identification system, streptococcal agglutination test kit, Gram stain, etc. were purchased from Guangdong Central Kai microbial reagent company. Methods: 80 stomatological stations of Guangdong Stomatological Hospital were taken from aseptic high-speed turbines and then taken for water sampling. The sampling time was 5 days after every Friday of 2007-05 / 10. Bacteriological tests were performed using conventional microbial isolation and identification techniques. MAIN OUTCOME MEASURES: Bacteria concentration in water sample and the detection rate of three kinds of bacteria including staphylococcus, streptococcus and actinomycetes. Results: The bacterial concentration of 5.15 × 10 ~ 1.59 × 103cfu / mL in water samples from oral cavity treatment units of oral comprehensive treatment units in clinical departments of Stomatology Hospital was 5.67 × 102cfu / mL on average, with the highest bacterial concentration in periodontal disease. The oral and maxillofacial surgery The lowest concentration of bacteria was in water samples, and there was significant difference among different departments (P = 0.000). There was also a significant difference in the detection rates of three bacteria among different departments (P = 0.000). Conclusions: The bacterial contamination of high speed turbine waterways in oral comprehensive treatment units in different departments of oral specialties is different. For those departments with low frequency of use of high speed turbines, it is suggested to include empty step on high speed turbine waterway in routine oral treatment, while for clinical Departments with more serious business exposures should also consider introducing additional infection control measures.