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以ＴＧＦ－β１在体外作用于人急性早幼粒白血病细胞株ＨＬ－６０细胞，通过免疫细胞化学染色等实验方法，观察ＴＧＦ－β１对ＨＬ－６０细胞株凋亡基因表达的影响。结果发现１μｇ／Ｌ的ＴＧＦ－β１体外作用２４ｈ后ＨＬ－６０细胞中Ｂｃｌ－２、Ｃ－ｍｙｃ基因的灰度值由２７．８０±３．１７和２０．８０±４．３７下调至３０．５６±２．５７和２３．４２±４．４５；作用７２ｈ后则分别下调至３５．０１±１．３４和３４．６１±１．６１。结果说明ＴＧＦ－β１对人急性早幼粒白血病细胞株的凋亡基因有显著下调作用，这种作用随时间的延长更加明显。
The effect of TGF-β1 on human acute myeloid leukemia cell line HL-60 cells was studied in vitro. The effect of TGF-β1 on apoptosis gene expression in HL-60 cell line was observed by immunocytochemical staining. The results showed that the gray levels of Bcl-2 and C-myc genes in HL-60 cells were downregulated from 27.80±3.17 and 20.80±4.37 to 30 after 1 μg/L of TGF-β1 in vitro. 56±2.57 and 23.42±4.45; after 72 h, they were down-regulated to 35.01±1.34 and 34.61±1.61, respectively. The results showed that TGF-β1 had a significant down-regulation of apoptosis genes in human acute promyelocytic leukemia cell lines, and this effect was more pronounced with time.