论文部分内容阅读
目的:研究没食子酸对嗜水气单胞菌以及温和气单胞菌的体外抑菌作用并进行对比。方法:没食子酸菌种原液,使用打孔法进行温和气单胞菌以及嗜水气单胞菌的体外抑菌试验,并将培养皿进行倒置培养,测定温和气单胞菌以及嗜水气单胞菌的抑菌圈直径,进行比较。结果:没食子酸对嗜水气单胞菌以及温和气单胞菌的MBC以及MIC均在250μg/m L左右,对比没有明显差异(P>0.05)。对嗜水气单胞菌以及温和气单胞菌的抑菌圈直径分别为(26.4±0.4)mm以及(24.2±0.2)mm,对比无明显差异,无统计学意义(P>0.05)。结论:没食子酸对于嗜水气单胞菌与温和气单胞菌均具有较好的抑菌效果,具有较大的开发价值。可将其应用到临床治疗治疗中来,与抗生素联用,不仅可以有效强化治疗效果同时也可以发挥抑菌作用,减少耐药菌的产生,避免药物残留,保护环境。
Objective: To study the inhibitory effect of gallic acid on Aeromonas hydrophila and Aeromonas sobria in vitro and to compare the results. Methods: Gallic acid bacteria stock solution, the use of perforated method for Aeromonas hydrophila and Aeromonas hydrophila bacteria in vitro antibacterial test, and Petri dishes inverted culture, determination of Aeromonas sobria and water vapor single Bacteriostatic zone diameter, compared. Results: The MBC and MIC of gallic acid to Aeromonas hydrophila and Aeromonas sobria were all about 250 μg / m L, with no significant difference (P> 0.05). The diameters of bacteriostatic circles of Aeromonas hydrophila and Aeromonas sobria were (26.4 ± 0.4) mm and (24.2 ± 0.2) mm, respectively, with no significant difference (P> 0.05). Conclusion: Gallic acid has good antibacterial activity against Aeromonas hydrophila and Aeromonas sobria, which has a great development value. It can be applied to clinical treatment and treatment, combined with antibiotics, not only can effectively enhance the therapeutic effect but also can play an antimicrobial effect, reduce the generation of resistant bacteria, to avoid drug residues and protect the environment.