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对疑似感染病毒的火龙果(Hylocereus undatus Britt.)进行高通量测序,分析病毒种类和相对含量,为火龙果病毒诊断和检测提供理论基础。测序reads经拼接和注释,共3 969条contig注释为病毒,包含44 354 147条reads。10条contig注释为CVX-NTU(JF937699),其含有的reads占所有病毒reads数量的85.18%;10条contig注释为ZyVX(AY366208),占所有病毒reads数量的4.97%;6条contig注释为SchVX(AY366207),占所有病毒reads数量的3.75%;6条contig注释为CVX(AF308158),占所有病毒reads数量的0.26%。拼接获得的CVX-NTU序列完全覆盖CVX-NTU(JF937699)基因组,核苷酸序列一致性为97%。编码的5个ORF核苷酸序列与CVX-NTU(JF937699)一致性为97%~98%,氨基酸序列一致性为97%~99%。拼接获得的CVX-NTU序列与CVX、ZyVX和SchVX的核苷酸序列一致性分别为78%、75%和70%。这是首次利用高通量测序检测火龙果病毒的报道。
High-throughput sequencing of the virus suspected Hylocereus undatus Britt. To analyze the virus type and relative content provided a theoretical basis for the diagnosis and detection of the dragon fruit virus. Sequencing reads were stitched and annotated, with a total of 3 969 contigs annotated as viruses containing 44 354 147 reads. 10 contigs annotated as CVX-NTU (JF937699), which contained 85.18% of the total number of all virus reads; 10 contigs annotated as ZyVX (AY366208), accounting for 4.97% of all virus reads; 6 contigs annotated as SchVX (AY366207), accounting for 3.75% of all virus reads; 6 contigs annotated as CVX (AF308158), accounting for 0.26% of all virus reads. The spliced CVX-NTU sequence completely covers the CVX-NTU (JF937699) genome with a nucleotide sequence identity of 97%. The nucleotide sequences of the 5 ORFs encoded by this program were 97% -98% identical to CVX-NTU (JF937699) and 97% -99% identical in amino acid sequence. The nucleotide sequences of the CVX-NTU sequences spliced with CVX, ZyVX and SchVX were 78%, 75% and 70%, respectively. This is the first report of using high-throughput sequencing to detect dragon fruit viruses.