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目的：研究广藿香油抑制人前列腺癌细胞PC3增殖效应，探讨其诱导细胞凋亡作用及机制。方法MTT法观察广藿香油对细胞抑制作用；透射电镜观察细胞凋亡形态；流式细胞仪检测细胞周期变化；比色法检测caspase-3，caspase-9活性改变。结果 MTT法检测提示广藿香油体外抑制PC3细胞增殖，且抑制作用呈剂量-时间依赖性；透射电镜显示药物作用后细胞出现凋亡；流式细胞仪检测提示广藿香油作用使G0/G1期细胞比例上升，与对照组比较有统计学意义（P＜0.05）；caspase-3，caspase-9活性随药物作用剂量、时间增加逐渐递增。结论广藿香油体外抑制PC3细胞增殖，其抗肿瘤作用机制与阻断细胞周期、诱导细胞凋亡和激活caspase-3和caspase-9活性有关。“,”Objective To investigate the mechanism of patchouli oil inhibiting the proliferation and inducing the apoptosis of human prostate cancer cells PC3. Methods The proliferation of cells was detected by MTT assay. The apoptosis morphology of cells was observed by transmission electron microscope; Cell cycle change was measured by flow cytometry;The activities of caspase-3 and caspase-9 were detected by colorimetric method. Results After treated with patchouli oil, apoptosis morphology of PC3 cells were found, the proliferation of PC3 cells was inhibited obviously with dose and time-dependent effects, the proportion of PC3 cells at G0/Gl phase was incresed(P<0.05), and the activities of caspase-3 and caspase-9 were enhanced with dose and time-dependent effects. Conclusion Patchouli oil inhibit the proliferation of PC3 cells which might be associated with G0/Gl phase arrest, cell apoptosis, and the activation of caspase-3 and caspase-9.