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目的掌握湖南省钩端螺旋体宿主动物主要种类、密度,以及感染率和感染类型等情况,为防治钩体病提供科学依据。方法按照2007年《湖南钩端螺旋体病监测实施方案》,在全省6个常规监测点和环洞庭湖区5个应急监测点系统开展钩体的宿主动物带菌率与带菌种群调查。以夹夜法在室外捕获鼠类,采用无菌操作采集鼠肾,Korthof方法进行培养;应用分群血清与新分离的钩端螺旋体作凝集试验确定菌群.应用交叉凝集素吸收试验或分型因子血来确定菌型。结果常规监测点有效培养459份,感染率为10.24%。湘潭县、沅江市、双峰县的感染率分别为3.81%、6.67%、44.19%(p<0.01)。黑线姬鼠、东方田鼠,小家鼠、褐家鼠的感染率分别为13.96%、12.50%、5.19%、4.55%(p<0.05)。浏阳市的蛙肾中培养出1株秋季型、3株澳洲型钩体。应急监测点有效培养296份,感染率为16.89%.其中沅江市、大通湖区、君山区、岳阳县、汉寿县的感染率分别是10.84%、24.39%、27.27%、41.46%、2.30% (p<0.0 1)。东方田鼠、褐家鼠、黑线姬鼠、黄毛鼠的感染率分别为28.36%、10.17%、9.26%、3.03%(p<0.01)。菌型主要是黄疸出血群赖型、赛罗群乌尔夫型。结论湖南省宿主动物密度和带菌率差异较大,以黄疸出血群和赛罗群为主,感染率较往年升高,必须加强综合防制措施.预防爆发和流行。
Objective To understand the main species and density of Leptospira host organisms in Hunan Province, as well as the infection rate and infection types, and provide a scientific basis for the prevention and control of leptospirosis. Methods According to the “Implementation Plan of Hunan Leptospirosis Surveillance in 2007”, host organism carrier rate and population survey of leptospira were conducted in 6 routine monitoring points in the province and 5 emergency monitoring points around Dongting Lake. Murmur was captured outdoors by the method of nocturnal capture and the murine kidney was harvested by aseptic technique and Korthof method was used for the culture.The colonies were identified by agglutination test with grouping sera and newly isolated leptospira.The bacterial agglutination test Blood to determine the mold. Results The routine monitoring points were effectively cultured 459, the infection rate was 10.24%. The infection rates in Xiangtan County, Yuanjiang City and Shuangfeng County were 3.81%, 6.67% and 44.19%, respectively (p <0.01). The infection rates of Apodemus agaete, Microtus fortis, Mus musculus and Rattus norvegicus were 13.96%, 12.50%, 5.19% and 4.55%, respectively (p <0.05). Liuyang frog kidney cultivated an autumn-type, 3 Australian-type hook body. The infection rate of Yuanjiang City, Datonghu District, Junshan District, Yueyang County and Hanshou County were 10.84%, 24.39%, 27.27%, 41.46% and 2.30% respectively, and the infection rate was 16.89% p <0.0 1). The infection rates of Microtus fortis, Rattus norvegicus, Apodemus agrarius and Rattus norvegicus were 28.36%, 10.17%, 9.26% and 3.03%, respectively (p <0.01). Mycobacterium jaundice is mainly based on the type of bleeding, Saierqun Wulff type. Conclusion The density of host animals and the rate of carrying bacteria in Hunan Province are quite different. The prevalence of infection is higher than that of those in the group of bleeding jaundice and cytoplasm, and the integrated prevention and control measures must be strengthened to prevent outbreak and epidemic.