文献曾报道应用免疫酶法、免疫荧光法及抗补体免疫荧光法测定宫颈癌细胞中单纯疱疹病毒(HSV)抗原。Nahmias用抗补体免疫荧光法检查浸润性宫颈癌细胞中单纯疱疹病毒抗原,其阳性率为54％,而正常宫颈细胞均为阴性。荧光标记在核、核周围区,细胞质和整个细胞。在抗补体免疫荧光法的基础上,曾毅等建立了抗补体免疫酶法,并用这种方法检查鼻咽癌细胞中的EB病毒核抗原。 本文应用抗补体免疫酶法检查了18例宫颈癌病人及21例同年龄组非癌妇女的宫颈脱落细胞,结果两组单纯疱疹病毒抗原阳性率分别为72.2％及28.6％,两者存在显著性差异,表明在我国宫颈癌与单纯疱疹病毒感染有关。 The literature has reported the use of immune enzyme method, immunofluorescence and anti-complement immunofluorescence assay of cervical herpes simplex virus (HSV) antigen. Nahmias anti-complement immunofluorescence examination of invasive herpes simplex virus in cervical cancer cells, the positive rate was 54%, while normal cervical cells were negative. Fluorescently labeled in the nucleus, perinuclear area, cytoplasm and whole cell. On the basis of anti-complement immunofluorescence method, Zeng Yi et al. Established an anti-complement immunoenzyme method and used this method to examine the Epstein-Barr virus nuclear antigen in nasopharyngeal carcinoma cells. In this paper, anti-complement immunoenzymatic examination of 18 cases of cervical cancer patients and 21 cases of non-cancerous women in the same age group of cervical exfoliated cells, the results of two groups of herpes simplex virus antigen positive rates were 72.2% and 28.6%, both significant Differences, indicating that in our country with cervical cancer and herpes simplex virus infection.