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以胰岛素为目标药物,以丝素(SF)和羟丙基壳聚糖(HPCS)为包药材料,复凝聚法制备SF-HPCS载药微球。采用红外光谱(FTIR)、扫描电镜(SEM)、X射线衍射(XRD)、热重分析(TGA)等对载药微球的结构、外部形貌及热性能等进行了表征。结果表明,所制备的载药微球表面密实,平均粒径22.4μm,呈正态分布;载药微球对胰岛素的包埋率达73.6%,大于HPCS载药微球(64.3%)及壳聚糖(CS)载药微球(57.1%);SF-HPCS载药微球在人工胃液中4h内累计释药率为21.3%,在人工肠液中24h内累计释药率达81.2%,48h累计释药率为92.2%,释放过程平稳、缓慢。
SF-HPCS drug-loaded microspheres were prepared by complex coacervation with insulin as the target drug and silk fibroin (SF) and hydroxypropyl chitosan (HPCS) as the drug-loading materials. The structure, external morphology and thermal properties of drug loaded microspheres were characterized by FTIR, SEM, XRD and TGA. The results showed that the prepared drug-loaded microspheres had a dense surface with an average particle size of 22.4μm and showed a normal distribution. The encapsulation efficiency of insulin-loaded microspheres was 73.6%, higher than that of HPCS drug-loaded microspheres (64.3%) and shell (CS) loaded microspheres (57.1%). The cumulative release rate of SF-HPCS loaded microspheres in artificial gastric juice was 21.3% within 4h, and the cumulative release rate in artificial intestinal juice was 81.2% within 48 h The cumulative release rate of 92.2%, the release process is stable and slow.