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目的探讨激活素A对小鼠纤维母细胞L929活性的调节作用。方法用不同剂量的激活素A刺激L929细胞,应用还原酶法分析细胞分泌一氧化氮(NO)的水平,RT-PCR法检测细胞诱导型一氧化氮合成酶(iNOS)、纤维连接蛋白(FN)和金属蛋白酶组织抑制物(TIMP)mRNA的表达,应用中性红检测细胞的吞饮活性,MTT法检测细胞的增殖活性。结果L929细胞经激活素A刺激后,与对照组细胞比较,分泌NO的水平明显升高,iNOS mRNA及FN mRNA的表达水平也升高,而TIMP mRNA的表达水平无明显改变。激活素A可以促进L929细胞的吞饮活性,但对L929细胞的增殖活性无影响。结论激活素A具有促进L929细胞分泌炎性介质和形成细胞外基质的作用,这可能是其促进炎症发展、诱导组织纤维化的原因。
Objective To investigate the regulatory effect of activin-A on the activity of L929 in mouse fibroblasts. Methods L929 cells were stimulated with different doses of activin A. The levels of nitric oxide (NO) secreted by cells were measured by reductase method. The expression of iNOS, fibronectin (FN) ) And tissue inhibitor of metalloproteinase (TIMP) mRNA expression were detected using neutral red swallowing activity of the cells, MTT assay of cell proliferation activity. Results Compared with the control group, L929 cells stimulated with activin A significantly increased the level of NO secretion and the expression of iNOS mRNA and FN mRNA, while the expression of TIMP mRNA did not change significantly. Activin A can promote the swallowing activity of L929 cells, but has no effect on the proliferation activity of L929 cells. Conclusions Activin A can promote the secretion of inflammatory mediators and extracellular matrix in L929 cells, which may be the reason of promoting the development of inflammation and inducing the fibrosis of tissues.