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目的探索采用下腔静脉缩窄术建立布-加综合征大鼠动物模型的可行性。方法将50只SD大鼠采用完全随机法随机分为实验组和假手术组,全麻开腹,分离肝上下腔静脉。实验组借鉴缩窄门静脉主干法,丝线环绕结扎肝后下腔静脉,使下腔静脉横截面积缩窄约80%。2组分别于术后不同时间点(第1、4、8、12周)行腹部彩超、肝功能、血常规及肝脏病理学检查。结果实验组在第4周时均出现肝后下腔静脉及主肝静脉梗阻,淤血性肝硬变,腹水,肝脾肿大,门静脉扩张,肝实质内交通支开放,而假手术组正常。模型建立后第4周时,实验组大鼠肝功能中ALB及血常规中WBC、PLT、RBC、HGB较假手术组明显降低(P<0.05),ALT、AST、AKP、TBIL、DBIL、TBA较假手术组明显升高(P<0.05)。结论下腔静脉缩窄术可成功建立布-加综合征大鼠动物模型。
Objective To explore the feasibility of establishing an animal model of Budd-Chiari syndrome by using inferior vena cava constriction. Methods Fifty SD rats were randomly divided into experimental group and sham operation group. The animals were anesthetized to open the upper and lower vena cava. The experimental group, referring to the narrowing of the main portal vein approach, ligature around the ligature ligation of the inferior vena cava, so that the cross-sectional area of the inferior vena cava narrowed about 80%. Two groups were performed at different time points (1st, 4th, 8th and 12th weeks) respectively. The abdominal ultrasonography, liver function, blood routine and liver pathology were performed. Results In the fourth week, the experimental group had posthepatic inferior vena cava and main hepatic vein obstruction, congestive liver cirrhosis, ascites, hepatosplenomegaly, portal vein dilatation, and hepatic parenchyma in the experimental group, while the sham group was normal. At the 4th week after model establishment, the levels of ALB, WBC, PLT, RBC and HGB in liver function of experimental group were significantly lower than those of sham operation group (P <0.05), ALT, AST, AKP, TBIL, DBIL, Than sham operation group was significantly higher (P <0.05). Conclusion Narrowing of inferior vena cava can successfully establish animal model of Budd-Chiari syndrome.