论文部分内容阅读
为了探讨昆明小鼠(Mus musculus)年龄相关性空间学习记忆能力改变及其与海马突触前囊泡蛋白1(synaptotagmin 1,SytⅠ)含量之间的关系。选取3个年龄段的昆明小鼠,①青年组:6月龄,28只;②中年组:11月龄,22只;③老年组:22月龄,17只。利用六臂辐射状水迷宫(RAWM)任务检测其空间学习记忆能力;制作组织微阵列,采用免疫组化技术检测SytⅠ在海马中的的表达;采用方差分析方法对六臂辐射状水迷宫实验参数和SytⅠ的相对含量进行统计学分析,使用Spearman秩相关检测这二者之间的相关性。结果发现,老年组昆明小鼠在学习期及记忆期的平均错误数及潜伏期均高于中年和青年鼠(P<0.05),中年昆明小鼠与青年鼠之间的差异不显著(P>0.05);老年组昆明小鼠在海马CA3区及齿状回(dentate gyrus,DG区)中SytⅠ的相对含量显著高于中年鼠和青年鼠(P<0.05);昆明小鼠海马CA3、DG区SytⅠ的相对含量与学习期和记忆期的错误数及潜伏期均成正相关(P<0.05)。由此推断,昆明小鼠可出现年龄相关性空间学习记忆能力降低,其海马CA3和DG的SytⅠ相对含量出现年龄相关性升高,海马SytⅠ升高可能与昆明小鼠年龄相关性空间学习记忆能力减退有关。
To investigate the relationship between age-related spatial learning and memory ability and the level of synaptotagmin-1 (SytⅠ) in the hippocampus of Mus musculus. Kunming mice of three age groups were selected, ① young group: 6 months, 28; ② middle-aged group: 11 months old, 22; ③ elderly group: 22 months old, 17. The six-arm Radial Water Labyrinth (RAWM) task was used to detect the spatial learning and memory ability. The tissue microarray was made and the expression of Syt I in the hippocampus was detected by immunohistochemistry. The six-arm radial water maze test parameters And Syt Ⅰ relative content of the statistical analysis, the use of Spearman rank correlation test between the two. The results showed that the average number of mistakes and incubation period of Kunming mice in old age group were higher than that of middle-aged and young mice (P <0.05), while there was no significant difference between middle-aged Kunming mice and young mice > 0.05). The relative content of SytⅠ in Kunming mice hippocampus CA3 and dentate gyrus (DG) group was significantly higher than that in middle aged mice and young mice (P <0.05) The relative content of SytⅠ in DG area was positively correlated with the number of errors and latency of learning and memory (P <0.05). It is concluded that age-related spatial learning and memory abilities decrease in age-dependent Kunming mice and the relative content of Syt I in hippocampal CA3 and DG is increased in age-related manner. Elevated Syt I in hippocampus may be related to age-related spatial learning and memory ability in Kunming mice Decreased.