论文部分内容阅读
为了了解人体鞭虫感染与年龄和体液免疫应答的关系,选择西印度群岛圣卢西亚一个总人口为3000人的村庄为试点。共观察122人,按4个年龄组分层抽样,其中0～4.9岁37人、5～9.9岁34人、10～30岁36人。>30岁15人。对每人收集粪便样本1g并静脉取血3ml。用Kato厚片法测定每克粪便中的虫卵数(EPG)。用鞭虫成虫抗原作ELISA测定血清中鞭虫抗体水平,所有血清在测定前经蛔虫成虫抗原孵育1h,以避免抗
To understand the relationship between human whipworm infection and age and humoral immune response, a pilot project was conducted in a village of Saint Lucia, West Indies, with a total population of 3,000. A total of 122 people were observed, stratified by four age groups, of which 37 were 0-4 years old, 34 were 5-9.9 years old and 36 were 10-30 years old. > 30 years old and 15 people. Collect 1 g of stool samples and 3 ml of venous blood for each person. The number of eggs per gram of stool (EPG) was determined using the Kato slab method. Serum whipworm antibody levels were measured by ELISA using whipworm adult antigens, and all sera were incubated with roundworm adult antigen for 1h before assay to avoid resistance