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目的检测分析TCE暴露小鼠肝功能、肝脏Treg细胞分泌的细胞因子IL-10含量以及脾脏Treg细胞数量的变化,探讨Treg细胞与TCE暴露所致肝功能损害的关系。方法雌性BALB/c小鼠饮水摄入2.5 mg/ml和5 mg/ml的TCE,在2、4、8、12周时采集外周血用全自动生化仪测定肝功能,ELISA检测肝组织匀浆IL-10含量,流式细胞仪检测脾脏Treg细胞数量。结果与对照组相比,TCE染毒组小鼠AST和ALT水平明显升高,2周和4周时差异有统计学意义(P<0.05),肝组织匀浆中IL-10含量则明显降低(4周时最低);脾脏Treg细胞数量也明显降低,2、4、8周时差异均有统计学意义(P<0.05);相关分析显示肝功能与肝组织中IL-10含量和Treg细胞数量均呈负相关,且有统计学意义(P<0.05)。结论 TCE暴露引起的肝脏损伤与体内Treg细胞数量减少或功能受损相关。
Objective To detect and analyze the liver function of mice exposed to TCE, the changes of cytokine IL-10 secreted by liver Treg cells and the number of splenic Treg cells, and to explore the relationship between Treg cells and liver damage caused by TCE exposure. Methods Female BALB / c mice were fed with 2.5 mg / ml TCE and 5 mg / ml TCE respectively. Peripheral blood was collected at 2, 4, 8, and 12 weeks. The liver function was measured by automatic biochemical analyzer. IL-10 content, flow cytometry Treg cell number. Results Compared with the control group, the levels of AST and ALT in TCE-treated mice were significantly increased at 2 and 4 weeks (P <0.05), and the levels of IL-10 in liver homogenate were significantly decreased (The lowest at 4 weeks). The number of spleen Treg cells was also significantly decreased, with significant difference at 2, 4, and 8 weeks (P <0.05). Correlation analysis showed that liver function and liver tissue IL-10 content and Treg cells The numbers were negatively correlated with statistical significance (P <0.05). Conclusion The liver damage caused by TCE exposure is related to the decrease of Treg cells or impaired function in vivo.