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目的:研究羟基红花黄色素A对大鼠脑缺血-再灌注损伤的保护作用及机制。方法:缺血前给予羟基红花黄色素A(2、4、8 mg/kg),舌下静脉给药3 d,末次给药1 h后制备大鼠大脑中动脉阻断短暂局灶性缺血模型。脑缺血2 h再灌注24 h后,用伊文思兰法(EB)测定血脑屏障的损伤程度。比色法测定缺血脑组织髓过氧化酶(MPO)和一氧化氮合酶(NOS)的活性,酶免法测定血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、白介素1β(IL-1β)的含量。结果:羟基红花黄色素A能减轻脑缺血-再灌注后神经损伤症状,抑制MPO和NOS活性,降低脑缺血-再灌注后血清中IL-6、IL-1β、TNF-α含量。结论:羟基红花黄色素A可通过抑制脑缺血-再灌注过程中炎症介质释放,降低脑缺血-再灌注所致脑损伤。
Objective: To study the protective effect and mechanism of hydroxysafflor yellow A on focal cerebral ischemia-reperfusion injury in rats. METHODS: Hydroxyrsalicyoride A (2,4,8 mg / kg) was given before ischemia and sublingual vein for 3 days. The middle cerebral artery was occluded for 1 h after the last administration to block the transient focal absence Blood model. After 24 h of reperfusion for 2 h after cerebral ischemia, the extent of BBB damage was measured by Evans blue method (EB). The activities of myeloperoxidase (MPO) and nitric oxide synthase (NOS) in ischemic brain tissue were measured by colorimetric method. The levels of serum tumor necrosis factor alpha (TNF-α), interleukin 6 (IL- Interleukin 1β (IL-1β) content. Results: Hydroxysafflor yellow A could relieve the symptoms of nerve injury after cerebral ischemia-reperfusion, inhibit the activity of MPO and NOS and decrease the content of IL-6, IL-1β and TNF-α in serum after cerebral ischemia-reperfusion. Conclusion: Hydroxysafflor yellow A can reduce brain injury induced by cerebral ischemia - reperfusion by inhibiting the release of inflammatory mediators during cerebral ischemia - reperfusion.