论文部分内容阅读
目的：探讨肿瘤与ＣｕＺｎＳＯＤ的关系。方法：用ＡＢＣ免疫组化法对１９例肝细胞癌及癌旁肝组织；４５例肺癌；７２例唾液腺良、恶性肿瘤及正常肝、肺、唾液组织切片中ＣｕＺｎＳＯＤ定位及初步定量。结果：ＣｕＺｎＳＯＤ分布在阳性细胞质和（或）核内，肝癌中ＣｕＺｎＳＯＤ阳性率（３１５８％）及染色强度明显＜正常肝组织＜癌旁组织。肺癌阳性率为５３３３％，肺鳞癌ＣｕＺｎＳＯＤ主要分布在细胞质，而肺腺癌主要分布在细胞质和核，二者之间有显著区别（Ｐ＜００１）。肺小细胞未分化癌阳性率为９０９％，显著低于其它类型肺癌（Ｐ＜００１）。唾液腺良、恶性肿瘤之间无明显差异。肝、肺及唾液腺三种不同组织来源的肿瘤ＣｕＺｎＳＯＤ含量差异很大。结论：肝、肺及唾液腺三种不同组织来源的肿瘤中ＣｕＺｎＳＯＤ含量差异很大，故不同组织类型的肿瘤抗氧化能力亦不同。其ＣｕＺｎＳＯＤ免疫定位及定量显示出病理学意义。
Objective: To investigate the relationship between tumor and CuZnSOD. Methods: The localization and preliminary quantification of CuZnSOD in 19 cases of hepatocellular carcinoma and adjacent liver tissue, 45 cases of lung cancer, 72 cases of salivary gland benign and malignant tumors, and normal liver, lung and saliva tissue sections by ABC immunohistochemistry. RESULTS: CuZnSOD was distributed in positive cytoplasm and/or nucleus. The positive rate of CuZnSOD (3158%) and the intensity of staining were significantly lower in normal liver tissue than that in normal liver tissue. The positive rate of lung cancer was 5333%. CuZnSOD was mainly distributed in the cytoplasm of lung squamous cell carcinoma, while the lung adenocarcinoma was mainly distributed in the cytoplasm and nucleus. There was a significant difference between them (P<001). The positive rate of lung small cell undifferentiated carcinoma was 9.9%, which was significantly lower than that of other types of lung cancer (P<001). There were no significant differences between salivary gland benign and malignant tumors. The CuZnSOD content of tumors from three different tissue sources of liver, lung and salivary gland varies greatly. Conclusion: The levels of CuZnSOD in tumors derived from liver, lung and salivary glands are very different. Therefore, the anti-oxidative capacity of tumors of different tissue types is also different. Its CuZnSOD immunolocalization and quantification showed pathological significance.