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采用γ射线诱变与高浓度羟脯氨酸（ＨＹＰ）离体选择的方法，获得抗羟脯氨酸（ＨＹＰ）锦橙悬浮细胞系，并再生植株。抗性细胞系比供体细胞系（对照）含有较高的脯氨酸（Ｐｒｏ），有较高的ＰＯＤ与ＣＡＴ活性及较强的抗ＨＹＰ能力，经冷驯化后抗性细胞系的抗寒性比供体细胞提高１．４℃，同时进一步加大了抗性细胞系在抗氧化酶ＳＯＤ、ＣＡＴ活性和脯氨酸（Ｐｒｏ）、可溶性糖含量及水势上与供体的差异。这表明，抗氧化酶活性的提高及渗透调节能力的加强与抗性系抗寒性的增强有关。
The method of γ-ray mutagenesis and high concentration of hydroxyproline (HYP) was used in vitro to obtain the hydroxyproline (HYP) suspension suspension cell line and to regenerate the plant. The resistant cell line contains higher proline (Pro) than the donor cell line (control), higher POD and CAT activity and stronger anti-HYP ability. After being cold-acclimated, the resistance of the resistant cell line to cold Compared with the donor cells, it increased by 1.4 ℃, and at the same time, the differences of the antioxidant enzymes SOD and CAT activity and proline (Pro), soluble sugar content and water potential were further enhanced. This indicates that the enhancement of antioxidant enzyme activity and osmotic adjustment is related to the increase of resistance to cold stress.