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目的:研究宫颈癌癌前病变及其癌组织中转化生长因子Ⅱ型受体(TGFBR2)的表达及临床意义。方法:取13例慢性宫颈炎、102例宫颈上皮内瘤变和167例宫颈癌组织,构建成四块组织芯片;采用链霉卵白素-过氧化物酶连结(SP)免疫组化方法,检测四块组织芯片中TGFBR2蛋白的表达,并分析其与宫颈癌临床病理特征和预后的关系。结果:成功对11例慢性宫颈炎、97例宫颈上皮内瘤变和164例宫颈癌组织中TGFBR2蛋白的表达进行检测。在慢性宫颈炎、宫颈上皮内瘤变、宫颈癌组织中,TGFBR2蛋白的阳性表达率分别为45.5%(5/11)、20.6%(20/97)、3.0%(5/164),差异均有统计学意义(P<0.05)。TGFBR2的表达与肿瘤的FIGO分期、浸润深度及淋巴结转移密切相关,即分期越晚、浸润深度越深、伴随淋巴结转移者,TGFBR2蛋白表达水平越低(P<0.05)。随访的164例宫颈癌患者中,TGFBR2阳性和阴性患者的5年生存率分别为72.4%和69.2%,两者差异有统计学意义(P<0.05)。多因素分析显示,TGFBR2表达情况和淋巴结转移情况为影响宫颈癌预后的独立因素。结论:TGFBR2表达下降与宫颈癌发生发展过程密切相关,可作为肿瘤治疗的靶点及预后的预测指标。
Objective: To study the expression of transforming growth factor type Ⅱ receptor (TGFBR2) in cervical precancerous lesions and its cancerous tissues and its clinical significance. Methods: Thirteen cases of chronic cervicitis, 102 cases of cervical intraepithelial neoplasia and 167 cases of cervical cancer were enrolled in this study. Four tissue microarrays were constructed. Immunohistochemistry with streptavidin-peroxidase (SP) The expression of TGFBR2 protein in four tissue microarray and its relationship with clinicopathological characteristics and prognosis of cervical cancer. Results: The expression of TGFBR2 protein in 11 cases of chronic cervicitis, 97 cases of cervical intraepithelial neoplasia and 164 cases of cervical cancer was successfully detected. In chronic cervicitis, cervical intraepithelial neoplasia and cervical cancer, the positive rates of TGFBR2 protein were 45.5% (5/11), 20.6% (20/97) and 3.0% (5/164), respectively There was statistical significance (P <0.05). The expression of TGFBR2 was closely related to the FIGO stage, depth of invasion and lymph node metastasis. The later the stage of invasion, the deeper the depth of invasion, the lower the expression of TGFBR2 protein (P <0.05). The follow-up 164 cases of cervical cancer patients, TGFBR2 positive and negative 5-year survival rates were 72.4% and 69.2%, the difference was statistically significant (P <0.05). Multivariate analysis showed that TGFBR2 expression and lymph node metastasis were independent prognostic factors of cervical cancer. CONCLUSION: The decreased expression of TGFBR2 is closely related to the occurrence and development of cervical cancer, which can be used as the target of tumor therapy and prognostic index.