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目的 探讨急性肺损伤 (ALI)时肺组织和血浆一氧化氮 (NO)、内皮素 - 1(ET - 1)和白细胞介素 8(IL - 8)水平的变化和作用。方法 采用间隔 2 4h两次注射大肠杆菌内毒素 (LPS)的方法 ,复制家兔内毒素性急性肺损伤模型。结果 ALI组血浆、肺组织匀浆及支气管肺泡灌洗液 (BALF)中NO、ET - 1和IL - 8水平显著升高 (P <0 0 1) ,BALF内中性粒细胞(PMN)明显增多 ,肺系数 (LPL)、肺水含量及通透指数均升高。血浆NO和IL - 8浓度于静脉注射LPS后 12 0min达峰值 ,ET - 1升高较早 ,6 0min达峰值 ,随后衰减 ,致伤 6h ,基本恢复接近正常水平。结论 NO、ET - 1和IL - 8是参与急性肺损伤不同时相的体液因子 ,三者共同参与ALI的发病
Objective To investigate the changes of lung tissue and plasma levels of nitric oxide (NO), endothelin - 1 (ET - 1) and interleukin - 8 (IL - 8) in acute lung injury (ALI) Methods The model of LPS-induced acute lung injury in rabbits was replicated by injecting E. coli lipopolysaccharide (LPS) twice every 24 hours. Results The levels of NO, ET - 1 and IL - 8 in plasma, lung homogenates and BALF were significantly increased in ALI group (P <0.01), and the levels of neutrophil (PMN) in BALF were significantly higher Increased lung coefficient (LPL), lung water content and permeability index increased. The concentrations of plasma NO and IL - 8 peaked at 120 minutes after intravenous injection of LPS. ET - 1 increased earlier and peaked at 60 minutes, then attenuated and injured for 6 hours, returning to normal level. Conclusions NO, ET - 1 and IL - 8 are the humoral factors involved in the different phases of acute lung injury. All three participate in the pathogenesis of ALI