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提取低剂量辐射(LDR)诱导蛋白,对其进行初步检测,观察其生物学活性、对有害理化因子造成细胞损伤的保护作用、对正常人及部分眼疾患者外周血淋巴细胞的刺激作用。小鼠给予 LDR 后,取脾脏细胞超声破碎,再经离心取得 LDR 诱导蛋白提取液,经凝胶过滤、电泳检测新生蛋白及其分子量。用 Lowry’s 法测定新增蛋白含量。用同位素掺入法观察 LDR 诱导蛋白生物学活性及影响其活性的因素、观察其对诸如紫外线(UV)、大剂量 Co γ 射线照 60射、热等有害因素造成细胞损伤的保护作用及其对正常人及部分眼疾患者外周血淋巴细胞转化的刺激作用。制备的 LDR 诱导蛋白提取液经凝胶过滤出现新增蛋白,其分子量为 76.9 KD±～110.0 KD±,产量为 613.33±213.42 μg /3×107个脾细胞。经 5～15 cGy LDR后 2～16 h 都可从小鼠脾脏细胞中提取得到 LDR 诱导蛋白并具有生物学活性,它可刺激离体小鼠脾脏细胞的转化,对某些有害理化因子造成的细胞损伤有明显的保护作用并具有刺激正常人及部分眼疾患者外周血 T,B 淋巴细胞转化的免疫增强作用。
Low-dose radiation (LDR) -induced protein was extracted and its activity was detected. The biological activity of the protein was observed, and the protective effect of harmful physiochemical factors on cell injury and the stimulation of peripheral blood lymphocytes in normal and some eye diseases were observed. Mice were given LDR, the spleen cells were sonicated, and then centrifuged to obtain LDR-induced protein extract, gel filtration, electrophoresis detection of newborn protein and its molecular weight. Lowry’s method was used to measure the protein content. The isotope incorporation method was used to observe the biological activity of LDR-induced protein and the factors that affect its activity. The protective effect of LDR on cell injury caused by harmful factors such as UV, high-dose Co γ-rays and so on Stimulation of peripheral blood lymphocyte transformation in normal and some eye diseases. The prepared LDR-induced protein extract gel filtration appeared new protein, the molecular weight of 76.9 KD ± ~ 110.0 KD ±, the yield of 613.33 ± 213.42 μg / 3 × 107 spleen cells. After 5 ~ 15 cGy LDR 2 ~ 16 h from the mouse spleen cells can be obtained from the LDR-induced protein and biological activity, it can stimulate the transformation of spleen cells in vitro, some of the harmful physical and chemical factors caused by the cells Injury has a significant protective effect and stimulates the normal and some eye disease patients with peripheral blood T, B lymphocyte transformation of immune enhancement.