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当变换柱子和流动相的条件,仍不能解决困难分离的时候,增加柱长或者让样品循环通过柱能获得效能的增加。本文介绍了让样品循环通过柱系统这样一种交替
When the conditions of the column and the mobile phase are changed, still difficult to solve difficult separation, increasing the column length or allowing the sample to circulate through the column can achieve an increase in potency. This article describes such an alternation of sample circulation through a column system