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目的观察K-ras基因在结直肠癌原发灶及肝转移灶的突变状态。方法采用富集PCR配对测序法检测46例(男35例,女11例)结直肠癌肝转移患者原发灶及肝转移灶K-ras基因突变状态,其中结肠癌肝转移21例(45.7%),直肠癌肝转移25例(54.3%)。结果46例中有18例(39.1%)原发灶K-ras基因为突变型,其中17例对应的肝转移灶K-ras基因为突变型,1例对应的肝转移灶K-ras基因为野生型。28例(60.9%)原发灶K-ras基因为野生型者中,26例对应的肝转移灶K-ras基因为野生型,2例对应的肝转移灶K-ras基因为突变型。统计分析表明,原发灶与肝转移灶K-ras基因突变状况差异无统计学意义(P=1.00)。结论转移性结直肠癌原发灶与肝转移灶的K-ras基因突变状态差异无统计学意义。
Objective To observe the mutation status of K-ras gene in primary and metastatic colorectal cancer. Methods Forty-six patients (35 males and 11 females) with colorectal cancer liver metastases were enrolled in this study. Mutations of K-ras gene were detected in primary and hepatic metastases. Among them, 21 (45.7% ), Liver metastases in rectal cancer in 25 cases (54.3%). Results K-ras gene was mutated in 18 cases (39.1%) of 46 cases, of which K-ras gene was mutated in 17 cases of liver metastases and K-ras gene in 1 case of liver metastases was Wild type. Among the 28 cases (60.9%), K-ras gene was wild type in the primary tumor, K-ras gene was wild type in the liver metastasis in 26 cases, and K-ras gene was in the liver metastasis in 2 cases. Statistical analysis showed no significant difference in K-ras gene mutation status between primary and hepatic metastases (P = 1.00). Conclusion There is no significant difference in K-ras gene mutation between primary and metastatic colorectal cancer.