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在快速病理诊断的过程中,冷冻切片制备的质量和速度决定了诊断的准确性和及时性,这就要求病理技术人员掌握熟练的切片技术及快速良好的染色方法。为了做到在更短的时间内染出优质的切片,我们改进了Carazzi苏木精染液,将整个冷冻切片染色时间从7~8 min缩短为3~4 min[1],以下加以介绍。1材料与方法1.1材料选取大理学院昆明附属医院2013-06—2014-03间术中快速冷冻组织标本700余例,分别为子宫肌瘤、甲状
During the rapid pathological diagnosis, the quality and speed of the frozen section preparation determines the accuracy and timeliness of the diagnosis, which requires the pathological technician to master the skilled sectioning technique and fast and good staining method. In order to dye high-quality chips in less time, we modified the Carazzi hematoxylin stain to shorten the total frozen section staining time from 7 to 8 min to 3 to 4 min [1], as described below. 1 Materials and Methods 1.1 Materials Selection Dali University Kunming Affiliated Hospital between 2013-06-2014-03 rapid intraoperative frozen tissue specimens more than 700 cases, respectively, uterine fibroids, thyroid