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作者用体内(Ehrlich腹水癌)试验法和试管内(Hela)及(Ehrlich腹水细胞)试验法,对蜈蚣等91种治癌中药的水提取物(Ⅰ)和甲醇提取物(Ⅱ)的抗癌活性进行了初步的筛选研究,方法结果如下。一、体内(Ehrlich腹水癌)试验: 向20g ddy雄性小鼠腹腔内移植Ehrlich腹水细胞(10~7Cells/只)五天后,腹腔注射给Ⅰ和Ⅱ。第四天开腹取出全部腹水,离心分离计测细胞容
The authors used the in vivo (Ehrlich ascites carcinoma) test and the in vitro (Hela) and (Ehrlich ascites) test methods to treat the cancer extracts of 91 Chinese herbal medicines including water extract (I) and methanol extract (II). A preliminary screening study was conducted on the activity. The results of the method are as follows. 1. In Vivo (Ehrlich Ascites Carcinoma) Assay: Ehrlich ascites cells (10-7 cells/piece) were intraperitoneally transplanted into 20 g ddy male mice and injected intraperitoneally into I and II. On the fourth day, all ascites were removed from the abdomen and measured by centrifugation.