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目的分析人乳头瘤病毒(HPV)永生化细胞系与Si Ha细胞系之间的蛋白质表达差异,探讨从HPV感染到宫颈癌发生间的变化。方法高效液相色谱(LC)与质谱(MS)联用对HPV永生化细胞系与Si Ha细胞系的蛋白质进行鉴定,然后通过蛋白质组学和生物信息学分析两种细胞系之间的蛋白表达,及一种新型翻译后修饰-琥珀酰化修饰的差异。结果在两种细胞系中,质谱鉴定到的蛋白总数为10 691个,琥珀酰化修饰位点共6 342个;非标定量分析共发现572种差异表达蛋白。HPV永生化细胞系中高表达的273种蛋白多集中在疾病的相关通路,而宫颈癌细胞系中高表达的299种蛋白多集中在代谢通路和核糖体通路。琥珀酰化修饰无差异,但发现一个新的琥珀酰化修饰位点——血清和糖皮质激素激酶1(SGK1)K41琥珀酰化。结论与HPV永生化细胞系相比,Si Ha细胞系代谢能力及翻译蛋白能力更强;而HPV永生化细胞中高表达蛋白则大多与疾病相关,且更易发生恶化。
Objective To analyze the difference of protein expression between human immortalized human papillomavirus (HPV) immortalized cell line and Si Ha cell line and to explore the change from HPV infection to occurrence of cervical cancer. Methods The proteins of HPV immortalized cell line and Si Ha cell line were identified by high performance liquid chromatography (LC) and mass spectrometry (MS), and the protein expression between the two cell lines was analyzed by proteomics and bioinformatics , And the difference between a novel post-translational modification-succinylation modification. Results In both cell lines, the total number of proteins identified by mass spectrometry was 10 691 and the number of succinylation sites was 6 342; 572 differentially expressed proteins were found by non-standard quantitative analysis. The high expression of 273 proteins in HPV immortalized cell lines mostly focused on disease-related pathways, whereas 299 highly expressed proteins in cervical cancer cell lines were concentrated in the metabolic and ribosomal pathways. There was no difference in succinylation modification, but a new succinylation modification site was found - serum and glucocorticoid kinase 1 (SGK1) K41 succinylation. Conclusions Compared with HPV immortalized cell line, Si Ha cell line has stronger ability of metabolism and translational protein. However, the highly expressed protein of HPV immortalized cell is mostly associated with disease, and is more likely to be exacerbated.