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运用聚丙烯酰胺凝胶电泳 ,对甜瓜属远缘杂种 (Cucumis× hytivus Chen & Kirkbride)与栽培黄瓜的回交自交群体共 4 6份材料进行了同工酶分析 ,结果表明 :群体内各单株间过氧化物酶酶谱差异较大 ,根据酶带数目及位置的差异可归为 6个类型 ,反映了群体内的遗传多态性。将回交自交群体与普通栽培黄瓜酶谱相比较时发现 ,群体比黄瓜新增了 4条带 ;群体的平均酶带数目 (7.8)比普通栽培黄瓜 (5 .0 )高出 5 6 .0 %。与此同时 ,在酶的活性上群体内也存在很大变异。这说明通过远缘杂交获得的新种对扩大黄瓜的遗传变异 ,改良黄瓜资源具有巨大的潜力。
By means of polyacrylamide gel electrophoresis, isozyme analysis was performed on 46 materials from backcross selfing populations of Cucumis × hytivus Chen & Kirkbride and cucumber cultivars. The results showed that isozymes There was a significant difference in the peroxidase zymogram between strains, which could be classified into six types according to the number and location of enzyme bands, which reflected the genetic polymorphism in the population. Comparing the backcrossed selfing population with that of common cucumber cultivars, we found that there were 4 new bands added to the cucumber population. The average number of bands in the population (7.8) was 56% higher than that of the common cucumber cultivars (5.0). 0%. In the meantime, there are also large variations in enzyme activity in the population. This shows that the new species obtained by distant hybridization to expand the genetic variation of cucumber, cucumber resources to improve has great potential.