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目的:探讨葛根素注射液对糖尿病骨质疏松的保护作用。方法:30只SD大鼠随机分为正常对照组、糖尿病组和糖尿病葛根素治疗组(n=10)。糖尿病组和糖尿病葛根素治疗组大鼠经腹腔注射链脲佐菌素(65mg/kg)制作糖尿病模型,成模后糖尿病葛根素治疗组每天腹腔注射葛根素注射液80mg/kg,正常对照组和糖尿病组注射等量生理盐水。成模6周后麻处死大鼠,分离各组大鼠的左侧股骨,于小动物放射成像系统上,测其近段骨密度,HE染色进行形态学观察。结果:成模6周后,糖尿病组骨密度显著低于对照组,糖尿病大鼠其股骨头表面成骨细胞与对照组比较显著减少,空骨陷窝明显增多,而葛根素治疗组上述指标均明显改善。结论:高糖可影响骨组织代谢及成骨细胞的增殖分化,造成糖尿病骨质疏松症,而葛根素对骨质疏松的进程可起到一定的保护作用。
Objective: To investigate the protective effect of puerarin on diabetic osteoporosis. Methods: Thirty SD rats were randomly divided into normal control group, diabetic group and diabetic puerarin treatment group (n = 10). Diabetic rats and diabetic Puerarin-treated rats were injected with streptozotocin (65mg / kg) intraperitoneally to induce diabetic rats. Puerarin-treated diabetic rats were injected intraperitoneally with puerarin 80mg / kg, Diabetic group was injected with normal saline. Six weeks later, the rats were anesthetized and the left femur of each group was isolated. The bone mineral density of proximal animals was measured by radioimmunoassay system. The morphological changes were observed by HE staining. Results: After 6 weeks, the BMD of diabetic group was significantly lower than that of the control group. Compared with the control group, the osteoblasts on the surface of the femoral head of diabetic rats were significantly decreased and the empty lacuna was significantly increased Significant improvement. Conclusion: High glucose can affect the metabolism of bone tissue and the proliferation and differentiation of osteoblasts, resulting in diabetic osteoporosis. Puerarin may play a protective role in the process of osteoporosis.