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目的研究低剂量辐射预先照射以及随后大剂量照射后小鼠胸腺细胞Ｔ细胞受体（ＴＣＲ）、ＣＤ３、ＣＤ４和ＣＤ８分子表达的变化。方法ＴＣＲ、ＣＤ３表达采用间接荧光流式细胞术检测，ＣＤ４、ＣＤ８表达采用双参数直接免疫荧光流式细胞术检测。结果实验结果表明：单纯１５ＧｙＸ射线全身照射后ＴＣＲ，ＣＤ３阳性细胞数以及ＣＤ４－Ｄ８－，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋细胞数明显减少，当１５ＧｙＸ射线全身照射前６小时预先照射００７５Ｇｙ时，可明显减轻其后１５Ｇｙ照射对ＴＣＲ＋，ＣＤ３＋，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋的损伤作用。表现为各亚组细胞数显著高于单纯１５Ｇｙ照射组。ＣＤ４－ＣＤ８－亚组的细胞数无明显变化。结论００７５ＧｙＸ射线全身照射能够诱导胸腺细胞ＴＣＲ＋，ＣＤ３＋，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋亚组细胞的适应性反应。
Objective To study the changes of T cell receptor (TCR), CD3, CD4 and CD8 expression in mouse thymocytes after pretreatment with low dose radiation and subsequent high dose irradiation. Methods TCR and CD3 expression were detected by indirect fluorescent flow cytometry. The expression of CD4 and CD8 were detected by two-parameter direct immunofluorescence flow cytometry. Results The experimental results showed that the number of TCR, CD3 positive cells and the number of CD4-D8-, CD4 + CD8 +, CD4 + CD8- and CD4-CD8 + cells decreased significantly after irradiation with 1.5Gy X-ray and were pre-irradiated 6 hours before the whole body irradiation At 0075 Gy, the injury of TCR +, CD3 +, CD4 + CD8 +, CD4 + CD8- and CD4-CD8 + after 15 Gy irradiation was significantly alleviated. The performance of each subgroup of cells was significantly higher than the simple 1 5Gy irradiation group. There was no significant change in the number of CD4-CD8-subgroups. Conclusion 0075 Gy X-rays can induce the adaptive response of TCR +, CD3 +, CD4 + CD8 +, CD4 + CD8- and CD4-CD8 + subsets in thymocytes.