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目的摘探讨白藜芦醇对力竭运动大鼠心肌细胞氧化应激、凋亡影响及机制。方法成年雄性SD大鼠随机分为5组:对照组、力竭运动组、白藜芦醇50、100、200 mg/kg组,检测大鼠心肌组织缺血缺氧面积、心肌组织超氧化物歧化酶(SOD)、丙二醛、Ca~(2+)-ATPase含量,TUNEL法检测心肌细胞凋亡率,Western blot法检测心肌细胞Bax和caspase-3蛋白表达。结果与对照组比较,力竭运动组大鼠心肌缺血缺氧面积[(112.27±48.27)μm~2]与心肌组织丙二醛含量[(3.98±0.75)nmol/mgpro]明显升高、心肌组织SOD活性[(30.54±4.29)U/mgpro]与Ca~(2+)-ATPase活性[(0.32±0.03)mol/(mg·h)]明显下降、心肌细胞凋亡指数[(23.4±2.3)%]与心肌组织Bax、caspase-3蛋白表达[(1.28±0.32)、(1.59±0.12)]明显升高;与力竭运动组比较,200 mg/kg白藜芦醇组大鼠心肌缺血缺氧面积[(22.12±10.22)μm~2]与心肌组织丙二醛含量[(1.65±0.11)nmol/mgpro]明显减少、心肌组织SOD、Ca~(2+)-ATPase活性[分别为(69.57±9.02)U/mgpro、(0.72±0.11)mol/(mg·h)]明显升高、心肌细胞凋亡指数[(10.4±1.3)%]与心肌组织Bax、caspase-3蛋白表达[(0.36±0.11)、(0.62±0.13)]明显降低(P<0.01);呈明显剂量效应关系。结论白藜芦醇可有效缓解大鼠力竭运动所致氧化应激状态,其机制可能与降低心肌组织Bax、caspase-3表达水平,减少心肌细胞凋亡有关。
Objective To explore the effect and mechanism of resveratrol on cardiomyocyte oxidative stress and apoptosis after exhaustive exercise in rats. Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups: control group, exhaustive exercise group, resveratrol 50, 100, 200 mg / kg group. The area of myocardial ischemia-reperfusion, the level of myocardial superoxide The contents of SOD, MDA and Ca ~ (2 +) - ATPase were measured. The apoptosis rate of cardiomyocytes was detected by TUNEL method. The protein expressions of Bax and caspase-3 were detected by Western blot. Results Compared with the control group, myocardial ischemia and anoxia area [(112.27 ± 48.27) μm ~ 2] and myocardial MDA content [(3.98 ± 0.75) nmol / mgpro] Compared with control group, the activity of SOD in [(30.54 ± 4.29) U / mgpro] and Ca ~ (2 +) - ATPase [(0.32 ± 0.03) mol / )% And myocardial tissue Bax, caspase-3 protein expression [(1.28 ± 0.32), (1.59 ± 0.12)] was significantly increased; compared with exhaustive exercise group, 200 mg / kg resveratrol group myocardial ischemia (22.12 ± 10.22) μm ~ 2 and MDA content in myocardium [(1.65 ± 0.11) nmol / mgpro] decreased significantly. The activities of SOD, Ca ~ (2 +) - ATPase in myocardium were (69.57 ± 9.02) U / mgpro, (0.72 ± 0.11) mol / (mg · h)], the apoptotic index [(10.4 ± 1.3)%] and the expression of Bax and caspase- (0.36 ± 0.11) and (0.62 ± 0.13)], respectively (P <0.01), showing obvious dose-effect relationship. Conclusion Resveratrol can effectively relieve the oxidative stress induced by exhaustive exercise in rats. The mechanism may be related to the decrease of the expression of Bax and caspase-3 in myocardium and the decrease of cardiomyocyte apoptosis.