目的　克隆并表达幽门螺杆菌粘附素 Alp A基因 .方法　提取幽门螺杆菌染色体基因 ,用 PCR方法扩增 alp A基因 ,将其克隆至表达载体 p ET- 2 2 b (+) ,转化大肠杆菌BL 2 1 (DE3) ,IPTG诱导表达 .结果　分离得到了 1 .5 kb的alp A基因片段 ,并在大肠杆菌中实现了该基因的高效表达 ,在 37℃诱导表达 3h后 ,表达产物占细菌总蛋白的 31 .9% .表达以可溶性蛋白和包涵体两种形式存在 ,其中主要是包涵体的形式 ,目的蛋白占不溶性蛋白的 64.8% .结论　幽门螺杆菌 alp A基因的克隆与表达为 Hp疫苗的研制打下了基础 Objective To clone and express the Helicobacter pylori adhesin Alp A gene.Methods The Helicobacter pylori chromosomal gene was extracted and the alp A gene was amplified by PCR and cloned into the expression vector p ET-2 2 b (+), which was then transformed into E.coli BL21 (DE3) and IPTG were induced in E.coli.Results The alp A gene fragment of 1.5 kb was isolated and expressed in Escherichia coli. After 3 hours of induction at 37 ℃, 31.9% of total protein.The expression existed in two forms of soluble protein and inclusion body, mainly in the form of inclusion body, the target protein accounted for 64.8% of insoluble protein.Conclusion Cloning and expression of Helicobacter pylori alp A gene is Hp Vaccine development laid the foundation