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目的 比较急性B淋巴细胞性白血病 (B ALL) ,非霍奇金淋巴瘤 (NHL)和慢性淋巴细胞性白血病(CLL)免疫表型表达的区别。方法 采用ABC AP法结合多种B淋巴细胞相关单克隆抗体 ,对 78例初发B ALL(均为CD-10 的病例 )、2 1例初发NHL和 2 5例初发CLL患者进行免疫表型检测。结果 CD-10 的B ALL患者的白血病细胞以CD19和HLA DR呈高阳性率表达 ;NHL患者的肿瘤细胞以CD2 0 和HLA DR呈高阳性率表达 ;CLL患者的白血病细胞除CD+ 19、CD+ 2 0 、CD+ 2 2 和HLA DR+ 等B淋巴细胞相关抗体表达外 ,主要还对CD5有很高的阳性率表达。结论 利用细胞免疫表型检测 ,对于临床上症状相似而仅以细胞形态学检查无法明确诊断的急性淋巴细胞性白血病、恶性淋巴瘤或慢性淋巴细胞性白血病 ,可能起到区别细胞类型 ,以协助诊断和治疗的作用
Objective To compare the differences of immunophenotypes between acute lymphoblastic leukemia (B ALL), non-Hodgkin’s lymphoma (NHL) and chronic lymphocytic leukemia (CLL). Methods A total of 78 patients with primary ALL (all CD-10 cases), 21 with primary NHL and 25 with primary CLL were immunized with ABC AP combined with various B lymphocyte-associated monoclonal antibodies Type testing. Results The leukemia cells of B ALL patients with CD-10 were highly positive for CD19 and HLA DR. The tumor cells of NHL patients were highly positive for CD20 and HLA DR. The leukemic cells of CLL patients except CD + 19 and CD + 2 0, CD + 2 2 and HLA DR + and other B lymphocyte-related antibody expression, the main CD5 also has a high positive rate. Conclusions The use of Cellular Immunophenotypic Tests may discriminate between cell types for diagnosis of acute lymphoblastic leukemia, malignant lymphoma or chronic lymphocytic leukemia that are clinically symptomatic but only diagnosed with cytomorphology alone And the role of treatment