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以玉米紧凑型自交系豫82、平展型自交系沈137为材料,利用同源克隆法成功克隆了与水稻Dwarf基因同源的玉米Dwarf2基因,该基因水稻上通过参与BR生物合成途径调控其株型形态建成。序列分析发现,玉米Dwarf2基因cDNA序列全长1 501bp,开放阅读框1 398bp,编码465个氨基酸。与水稻Dwarf基因的同源度达84%。实时荧光定量PCR分析表明,豫82、沈137中Dwarf2基因表达趋势基本一致,3～4叶期表达量低,8～12叶期处于表达高峰,13叶期后表达量逐渐下降。沈137中表达量较豫82高;就不同器官来说,沈137在叶片、叶枕、叶鞘、茎尖等器官中表达量较豫82高,其中,叶枕处ZmDwarf2表达量相对较高,而叶鞘上ZmDwarf2表达量相对较低。可以推测,基因ZmDwarf2可能参与玉米中BR生物合成途径,进而调控其相关株型形态建成。
The compact maize inbred line Yu 82 and flat inbred line Shen 137 were used as materials to clone the Dwarf2 gene homologous to the Dwarf gene in rice by homologous cloning. The gene was regulated by participating in the BR biosynthesis pathway Its plant type morphology is completed. Sequence analysis showed that the cDNA sequence of maize Dwarf2 gene was 1 501bp in length and 1 398 bp in open reading frame, encoding 465 amino acids. Its homology with rice Dwarf gene is 84%. Real-time quantitative PCR analysis showed that the expression trends of Dwarf2 gene in Yu 82 and Shen 137 were basically the same. The expression of Dwarf2 gene was low in the 3rd to 4th leaf stage and peaked in the 8th to 12th leaf stage, and gradually decreased after the 13th leaf stage. In Shen 137, the expression of ZmDwarf2 was higher than that of Yu 82. In different organs, the expression of Shen 137 in leaf, leaf pillow, leaf sheath, While the expression of ZmDwarf2 on leaf sheath is relatively low. It can be speculated that the gene ZmDwarf2 may be involved in BR biosynthesis pathway in maize, and then regulate its related plant type morphogenesis.