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用DIFA初筛检测197例武汉地区非选择性孕妇，其弓形虫抗体阳性率为8．12％（16／197）；阳性者用IFT—IgG复查，阳性符合率为93．75％，即阳性率为7.61％（15／197），15例阳性者的滴度分别为1：164例，1:649例，≥1：10242例；DIFA阳性者还用Toxo－IgG／IgA／IgM－ISAGA检测，有2例IgA和IgM均为阳性。其中1例IgA1：64（＋）、IgM1：16384（＋）为急性弓形虫感染件发葡萄胎。DIFA是一种敏感性高、特异性强、操作简便适用于孕妇弓形虫感染初筛的好方法，为达到优生优育监测弓形虫感染的目的，而采用DIFA、IFT－IgG、Toxo－IgG／IgA／IgM联合测试是较好的方案。
The positive rate of Toxoplasma gondii antibody in 197 cases of non-selective pregnant women in Wuhan was 8.12% (16/197) by DIFA screening. The positive rate was 93.75% with IFT-IgG, the positive rate was positive The titers of 15 positive cases were 1: 164 cases, 1: 649 cases, ≥1: 10242 cases. The positive rates of Toxo-IgG / IgA / IgM-ISAGA , 2 cases of IgA and IgM were positive. One case of IgA1: 64 (+), IgM1: 16384 (+) for the acute Toxoplasma gondii infection mole. DIFA is a sensitive, specific and easy-to-use method for screening Toxoplasma gondii in pregnant women. To achieve the purpose of prenatal and postnatal care to monitor Toxoplasma gondii infection, DIFA, IFT-IgG and Toxo-IgG / IgA / IgM joint test is a better solution.