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目的观察β1整合素反义寡核苷酸(ASODN)对人胰腺癌BXPC-3细胞体外侵袭力的影响。方法采用脂质体将不同浓度β1整合素ASODN转染到人胰腺癌BXPC-3细胞中,逆转录-聚合酶链反应(RT-PCR)、Western印迹法和Transwell侵袭室方法分别检测细胞β1整合素mR- NA、蛋白表达水平及体外侵袭能力。结果与对照组相比,32～128mg/L ASODN转染后可抑制81整合素mRNA和蛋白的表达(P＜0．05),64mg/L ASODN转染使BXPC-3细胞体外侵袭力明显下降(P＜0．05)。结论靶向β1整合素的ASODN可有效抑制其在人胰腺癌BXPC-3细胞中的表达,并降低癌细胞体外侵袭力。
Objective To investigate the effect of β1 integrin antisense oligonucleotide (ASODN) on invasiveness of human pancreatic cancer BXPC-3 cells in vitro. Methods The different concentrations of β1 integrin ASODN were transfected into BXPC-3 cells by lipofectamine. The expression of β1 integrin was detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and Transwell invasion assay MR-NA, protein expression and in vitro invasiveness. Results Compared with the control group, the expression of 81 integrin mRNA and protein was inhibited by 32 ~ 128 mg / L ASODN transfection (P <0.05). The transfection of ASODN at 64 mg / L significantly decreased the invasiveness of BXPC-3 cells in vitro (P <0.05). Conclusion ASODN targeting β1 integrin can effectively inhibit its expression in human pancreatic cancer BXPC-3 cells and reduce invasiveness of cancer cells in vitro.