论文部分内容阅读
目的 :研究三环类抗抑郁药地昔帕明 (DMI)对脑胶质瘤C6细胞增殖的调控作用 ,并探讨与临床常用治疗脑瘤的化疗药物替尼泊苷 (VM 2 6 )合用的协同效应。方法 :采用MTT比色法测定大鼠脑胶质瘤C6细胞增殖抑制作用和流式细胞术 (FCM)进行细胞周期分析。结果 :DMI (10— 80 μmol/L)对C6细胞的增殖具有明显的抑制作用 ,呈浓度时间依赖关系 ,药物作用 2 4h的IC50 (95 %置信区间 )为 2 0 7(17 3— 2 4 2 ) μmol/L。DMI (2 0 μmol/L)可使G0 -G1期细胞增加 (P <0 0 5 )而S期细胞减少 (P <0 0 5 ) ,G2 期细胞则无改变。不同浓度DMI和VM 2 6合用显示明显协同效应 (Q =0 72 )。结论 :DMI体外对C6细胞增殖具有浓度时间依赖性抑制作用 ,使细胞阻滞于G0 -G1期 ;与VM 2 6合用呈协同效应
OBJECTIVE: To study the effect of the tricyclic antidepressant decitabine (DMI) on the proliferation of glioma C6 cells, and to investigate the use of the chemotherapy drug commonly used in the treatment of brain tumors, teniposide (VM 26). Synergies. Methods: MTT colorimetric assay was used to determine the inhibitory effect of the proliferation of rat glioma C6 cells and flow cytometry (FCM) was used to analyze the cell cycle. RESULTS: DMI (10-80 μmol/L) significantly inhibited the proliferation of C6 cells in a concentration-time-dependent manner. The IC50 (95% confidence interval) of 24 h after drug exposure was 2 0 7 (17 3 - 2 4 2) μmol/L. DMI (20 μmol/L) increased G0-G1 phase cells (P <0 05) and S phase cells (P <0 05), while G2 cells did not change. Different concentrations of DMI and VM 26 combined showed significant synergistic effects (Q = 0 72). Conclusion : DMI has a time-dependent inhibition effect on the proliferation of C6 cells in vitro, resulting in cell arrest in the G0-G1 phase; a synergistic effect with the combination of VM 26