1997～ 1999年对甘蓝、花椰菜转Bt抗虫基因R0 代及R1代植株离体叶片进行了抗虫检测试验。结果表明 ,使用小菜蛾 1龄幼虫 ,每9cm圆形叶片接种 2 0条 ,在 2 0～ 2 2℃培养 5～6d ,以校正死亡率和叶片损害程度综合评估抗虫性 ,可取得有效的结果 ,此检测方法比较简便 ,可用于单株抗虫性的初步筛选。 From 1997 to 1999, the insect-resistant test was carried out on the leaves of R0 generation and R1 plants of Bt transgenic cabbage and cauliflower. The results showed that the first instar larvae of Plutella xylostella were used to inoculate 20 rounds of 9 cm round leaves and cultured for 5 to 6 days at 20 to 22 ° C to effectively evaluate the mortality and the extent of leaf damage for evaluating insect resistance Of the results, this test method is relatively simple, can be used for preliminary screening of insect resistance.