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目的:分析无偿献血者乙型肝炎表面抗原(HBsAg)筛查反应性标本的确证试验结果,以探索血站实施HBsAg筛查的实际效果。方法:随机收集无偿献血者HBsAg筛查反应性标本365例,采用中和试验法对配套HBsAg诊断试剂盒(酶联免疫法)检测结果呈重复阳性的标本进行确证试验。结果:365例HBsAg筛查反应性血液标本进行中和试验,其中ELISA方法检测HBsAg确证阳性率为51.23%;双试剂ELISA反应性与单试剂反应性标本确证试验阳性率的差异有统计学意义(P<0.05);初次献血者与重复献血者确证试验阳性率的差异、男女确证阳性率的差异和不同年龄组确证阳性率的差异均有统计学意义(P<0.05)。结论:对于筛查结果为单试剂反应性和S/CO<阳性预期值的献血者、重复献血者、低龄献血者更加适宜建立归队机制。
Objective: To analyze the confirmatory test results of HBsAg screening in unpaid blood donors to explore the practical effect of blood screening for HBsAg screening. Methods: One hundred and sixty-six HBsAg-positive samples were collected randomly from non-compensated donors. The neutralization test was used to confirm the test results of HBsAg diagnostic kit (enzyme-linked immunosorbent assay). Results: 365 cases of HBsAg-reactive blood samples were tested for neutralization. The positive rate of HBsAg confirmatory test by ELISA was 51.23%. There was significant difference between the positive rate of double-reagent ELISA and that of single-reagent reactive samples P <0.05). The positive rate of confirmatory test in primary blood donors and repeat blood donors showed significant difference (P <0.05) between the positive rate of male and female confirmed positive rate and the positive rate of positive rate in different age groups. CONCLUSIONS: For blood donors, repeat donors, and younger blood donors whose screening results are single-reagent reactivity and positive S / CO predictions, it is more appropriate to establish a rehoming mechanism.