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目的研究低强度激光(选用激光的波长为650nm、强度为5mW)血管外照射对兔实验性主动脉粥样硬化病理学过程的影响。方法共选取雄性新西兰大白兔36只,经先期基础喂养1周后给予高脂饮食；然后将其随机分为照射组(给予激光血管外照射)、药物组(给予辛伐他汀治疗)、综合组(给予激光及药物治疗)及对照组。各组动物于第60天实验结束时抽血行血脂测定；于第61天时将其处死,每只动物均切取主动脉组织进行大体及镜下观察,并将各组实验动物动脉粥样硬化程度按正常、轻、中、重度进行评定。结果实验开始前,4组实验兔血脂水平间差异无统计学意义(P＞0．05)；分别经高脂饲料喂养60d后,照射组、药物组及综合组TG、LDL-C含量与对照组比较,差异均有统计学意义(P＜0．05)；照射组、综合组HDL-C含量与对照组比较,差异亦有统计学意义(P＜0．01)；另外还发现,实验兔主动脉粥样硬化的病理学改变与其血脂变化程度相对应；各治疗组实验动物主动脉粥样硬化程度均以轻度为主,而对照组则以中、重度为主,综合组均为轻度改变,如照射组与药物组中度改变只有1只,对照组不但有1只为重度改变,另还有3只为中度改变。结论本研究结果表明,无论是单独给予低强度激光照射或他汀类药物治疗或是两者联合治疗,对防治血脂异常所致的动脉粥样硬化均有一定疗效,其中以两者联用效果较佳。
Objective To study the effect of low-intensity laser (laser wavelength 650nm, intensity 5mW) on pathological process of experimental aortic atherosclerosis in rabbits. Methods Thirty-six male New Zealand white rabbits were selected and given a high-fat diet for one week after pre-feeding. Then they were randomly divided into irradiation group (laser irradiation), drug group (simvastatin treatment group) (Given laser and drug treatment) and control group. The animals in each group were drawn blood for blood test at the end of the experiment on the 60th day. The animals were sacrificed on the 61st day. The aorta tissues of each animal were observed for gross and microscopic observation, and the degree of atherosclerosis By normal, light, moderate and severe assessment. Results Before the start of the experiment, there was no significant difference in serum lipids between the four experimental groups (P> 0.05). After 60 days of high-fat diet, TG and LDL-C levels in the irradiated group, (P <0.05). Compared with the control group, HDL-C levels in the irradiation group and the combination group were also significantly different (P <0.01). In addition, it was also found that the experimental The pathological changes of aortic atherosclerosis in rabbits corresponded to the changes of blood lipids. The severity of aortic atherosclerosis in experimental group was mild, while that in control group was moderate and severe Mild changes, such as the irradiation group and the drug group only a moderate change in the control group, not only a severe change, while the other three were moderate changes. Conclusion The results of this study show that either low-intensity laser irradiation alone or statin therapy or a combination of both have some effect on the prevention and treatment of atherosclerosis caused by dyslipidemia, of which the combination of the two is more effective good.