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目的:建立鳖甲抗肝纤维化有效部位的高效毛细管电泳(HPCE)指纹图谱。方法:运用毛细管区带电泳法,75μm×57cm未涂层石英毛细管,运行缓冲液为20mmol/L磷酸氢二钠-100mmol/L三羟甲基氨基甲烷(1∶1,1mol/LNaOH调pH值至12.0),电压为18kV,检测波长为254nm,柱温20℃,建立了10批不同产地鳖甲有效部位的指纹图谱。结果:标定了16个共有峰,各色谱峰分离较好,方法学考察符合有关要求。结论:HPCE指纹图谱分析法准确可靠,为鳖甲抗肝纤维化有效部位的质量控制提供了参考依据。
Objective: To establish a high performance capillary electrophoresis (HPCE) fingerprint of anti-hepatic fibrosis effective fraction. Methods: Using capillary zone electrophoresis, 75μm × 57cm uncoated quartz capillary, running buffer 20mmol/L disodium hydrogen phosphate-100mmol/L trimethylolaminomethane (1:1, 1mol/LNaOH to adjust the pH value To 12.0), the voltage was 18 kV, the detection wavelength was 254 nm, and the column temperature was 20°C. Ten lots of fingerprints of effective parts of the armor from different origins were established. Results: 16 common peaks were demarcated, each chromatographic peak was well separated, and methodological investigations met the relevant requirements. Conclusion: The HPCE fingerprinting method is accurate and reliable, which provides a reference for the quality control of the effective site of anti-hepatic fibrosis.