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目的分析胸腺细胞对胸腺上皮细胞分泌干扰素的影响。方法用胸腺细胞和胸腺基质细胞混合培养模型，测定ＩＦＮ活性并进行类型鉴别。结果胸腺细胞可以促进小鼠胸腺上皮细胞系（ＭＴＥＣ１）产生Ⅰ型ＩＦＮ，胸腺细胞和胸腺基质细胞以４０１的比例培养时，促进作用最强。ＭＴＥＣ１细胞经抗ＩＣＡＭ－１抗体作用后，分泌Ⅰ型ＩＦＮ增多。用放线菌酮预处理ＭＴＥＣ１细胞后，胸腺细胞和抗ＩＣＡＭ－１抗体则不再影响ＭＴＥＣ１细胞产生ＩＦＮ。结论胸腺细胞与基质细胞的相互作用，可由粘附分子ＩＣＡＭ－１／ＬＦＡ－１介导。胸腺细胞能促进ＭＴＥＣ１细胞合成ＩＦＮ。
Objective To analyze the influence of thymic cells on the secretion of interferon by thymic epithelial cells. Methods Thymus cells and thymus stromal cells mixed culture model, determination of IFN activity and type identification. Results Thymocytes promoted the production of type I IFN in mouse thymic epithelial cell line (MTEC1). When cultured in the ratio of 401, thymocytes and thymocytes were the strongest promoters. MTEC1 cells by anti-ICAM-1 antibody, the secretion of type I IFN increased. After pretreatment of MTEC1 cells with cycloheximide, thymocytes and anti-ICAM-1 antibodies no longer affect the production of IFN in MTEC1 cells. Conclusion The interaction between thymocytes and stromal cells can be mediated by the adhesion molecule ICAM-1 / LFA-1. Thymocytes can promote MTEC1 cells to synthesize IFN.