目前，对弓形虫速殖子的分离、净化方法较多，即使同一种方法，各家意见不一。为选择一种适宜的方法，使之简单易行、收获率高、敏感性、特异性均较好的方法，我们特进行5种常用方法的比较，以期获分离、净化速殖子的合适手段。材料和方法一、速殖子分离净化方法取昆明小鼠300 Currently, Toxoplasma gondii tachyzoites separation and purification methods are more, even if the same method, different opinions. In order to choose a suitable method to make it simple, high yield, sensitivity and specificity are better methods, we conducted a special comparison of five commonly used methods in order to obtain the appropriate means of separation and purification of tachyzoites . Materials and methods First, tachygotic separation and purification method Take Kunming mice 300