论文部分内容阅读
目的:建立喉症丸中华蟾酥毒基和脂蟾毒配基的含量测定方法.方法:采用高效液相色谱法进行测定, Thermo C18色谱柱 (250mm×4.6mm, 5μm), 流动相为乙腈-0.5％磷酸二氢钾 (用磷酸调节pH=3.2) (40:60), 流速1.0mL·min-1, 检测波长296nm, 柱温40℃, 进样体积10μL.结果:该方法系统适应性良好, 华蟾酥毒基、脂蟾毒配基分别在48.70～113.64μg·mL-1 (r=0.999 8) 和9.23～21.53μg·mL-1 (r=0.999 8) 范围内呈良好的线性关系, 平均加样回收率分别为101.1％ (RSD=0.6％, n=6) 、100.6％ (RSD=0.1％, n=6), 该方法精密度良好, 流速和柱温耐用性考察均符合要求.结论:该方法简便准确、灵敏、重复性好, 适用于喉症丸中蟾酥的质量控制.“,”Objective:To establish a determination method of cinobufagin and resibufogenin in Houzheng Pills.Methods:High Performance Liquid Chromatography (HPLC) was used for the content determination, Thermo C18 column (250 mm×4.6 mm, 5μm) was used with acetonitrile-0.5％ monopotassium phosphate (Adjusted PH to 3.2 with phosphric acid) (40:60) as the mobile phase, the flow rate was 1.0 mL·min-1, the detection wavelength was 296 nm, the column temperature was 40℃, the sample size was 10μL.Results:The method had a good system suitability, cinobufagin and resibufogenin showed good linear relationships within the ranges of 48.70～113.64μg·mL-1 (r=0.999 8) and 9.23～21.53μg·mL-1 (r=0.999 8), and the average recoveries were 101.1％ (RSD=0.6％, n=6) and 100.6％ (RSD=0.1％, n=6), respectively.The method had good precision, and the durability of flow-rate and column temperature meet the requirement.Conclusion:The method is accurate, reliable and simple.It can be used for the quality control of Houzheng Pills.