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一、愈伤组织与细胞悬浮液培养本方法对非两性结合的植物杂交:如应用化学诱变剂、细胞移接及细胞融合等技术可以得到更好的效果。例如经秋水仙素处理后,由甘蔗组织及细胞培养或由染色体嵌合而培养的蔗株,可以诱发和控制甘蔗无性杂交世代的变异。染色体嵌合幼株的变异在无性系“H50-7209”可以观察到染色体的变异和细胞悬浮液的培养,它证明通过愈伤组织培养的幼株有分化。由一个无性系采取愈伤组织培养,可以得到一连串非整倍体。如
First, the callus and cell suspension culture This method of non-bisexual plant hybridization: such as the use of chemical mutagens, cell migration and cell fusion technology can get better results. For example, after being treated with colchicine, sugarcane tissue and cell culture or by chromosome mosaic culture can induce and control the variation of sugarcane clones. Chromosome Chimerism Variants Chromosomal variation and cell suspension cultures can be observed in clones “H50-7209”, demonstrating differentiation of young plants cultured by callus. Callus culture is performed by one clones, and a series of aneuploidies can be obtained. Such as